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Am J Perinatol 2024; 41(08): 1069-1076
DOI: 10.1055/s-0042-1748324
Original Article

Therapeutic Role of Astaxanthin and Resveratrol in an Experimental Rat Model of Supraceliac Aortic Ischemia-Reperfusion

Authors

  • Dilek Dilli 

    1   Department of Neonatology, University of Health Sciences of Turkey, Dr. Sami Ulus Maternity and Children Training and Research Hospital, Ankara, Turkey

  • İrfan Taşoğlu

    2   Department of Cardiovascular Surgery, University of Health Sciences of Turkey, Türkiye Yüksek İhtisas Eğitim ve Araştırma Hastanesi, Ankara City Hospital, Ankara, Turkey

  • Eyüp Sarı

    3   Department of Management, Gülhane Faculty of Medicine/Ankara Provincial Health Directorate, University of Health Sciences of Turkey, Public Hospitals Services Presidency, Ankara, Turkey

  • Hasan Akduman

    1   Department of Neonatology, University of Health Sciences of Turkey, Dr. Sami Ulus Maternity and Children Training and Research Hospital, Ankara, Turkey

  • Nihat Yumuşak

    4   Department of Pathology, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, Turkey

  • Naim Boran Tümer

    2   Department of Cardiovascular Surgery, University of Health Sciences of Turkey, Türkiye Yüksek İhtisas Eğitim ve Araştırma Hastanesi, Ankara City Hospital, Ankara, Turkey

  • Salih Salar

    5   Department of Laboratory, Saki Yenilli Experimental Animals Production Laboratory, Ankara, Turkey

Funding This project was supported by the Scientific Research Projects (BAP) Commission of Health Sciences University of Turkey (Research project no.: 2020/060). We declare that the funders did not have a role in the study design, data collection, and analysis, decision to publish, or preparation of the manuscript.
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Abstract

Objective The aim of the study is to investigate the therapeutic effects of astaxanthin (AST) and resveratrol (RVT) on multiorgan damage in an animal model of the supraceliac aortic ischemia-reperfusion (I/R).

Methods In this study, 28 rats (n = 7/group), 200 to 250 g in weight, were randomized to four groups (1: Sham, 2: Control + I/R, 3: AST + I/R, and 4: RVT + I/R). Following the abdominal incision, aortic dissection was performed in the sham group without injury. Other groups underwent I/R injury via supraceliac aortic clamping (20 minutes) and reperfusion. The rats were administered olive oil (3 mL/kg) orally for 2 weeks before and 1 week after the laparotomy. Additionally, oral AST (10 mg/kg) or RVT (50 mg/kg) was given to the study groups. All rats were sacrificed on the 3rd week of the experiment after blood samples were taken for analysis. Multiple rat tissues were removed.

Results We found that RVT increased total antioxidant status (TAS) and superoxide dismutase (SOD) levels, and decreased total oxidant status (TOS), oxidative stress index (OSI), myeloperoxidase (MPO), and malondialdehyde (MDA) levels, while AST increased the levels of TAS, decreased TNF-α, MDA, TOS, and OSI (p <0.05). Pathological investigations of the rat tissues revealed that both AST and RVT ameliorated tissue damage and apoptosis.

Conclusion Our study suggests that AST and RVT might show therapeutic effects against oxidative tissue damage and apoptosis in an animal model of aortic I/R. Further studies are required.

Key Points

  • Major congenital heart diseases are at high risk of multiorgan damage.

  • Re-establishment of blood flow may result in ischemia-reperfusion (I/R) injury.

  • Astaxanthin and resveratrol may have therapeutic effects against I/R injury.

Keywords

antioxidants - aorta - ischemia/reperfusion - rat

Authors' Contributions

D.D. did the data curation, formal analysis, resources, software, writing-original draft, writing-review and editing. İ.T. contributed toward data curation, formal analysis, investigation, methodology, resources, writing-original draft, writing-review and editing. E.S. did the conceptualization, resources, validation, writing-review and editing. H.A. did the formal analysis, investigation, methodology, resources, software, validation, writing-original draft, writing-review and editing. N.Y. did the formal analysis, investigation, methodology, resources, software, validation, visualization, writing-original draft. N.B.T. contributed toward conceptualization, data curation, formal analysis, investigation, methodology, project administration, resources, software, supervision, validation, visualization, writing-original draft, writing-review and editing. S.S. did the formal analysis, investigation, methodology, resources, software, supervision, validation, visualization, writing-original draft.




Publication History

Received: 02 January 2022

Accepted: 28 February 2022

Article published online:
29 May 2022

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